Paleogenetics Lab

The molecular genetic laboratories for environmental and paleogenetics are specialized to analyze either organismic material (e.g. individual terrestrial plants) and recent environmental DNA or ancient environmental DNA from lake sediments archives or permafrost deposits. We apply DNA metabarcoding and metagenomics to investigate spatial and temporal biodiversity with the aim to characterize the taxonomic and phylogenetic composition of assemblies and their changes in Arctic terrestrial ecosystems.

The laboratories are divided into general genetic labs, which are separated in DNA isolation and Post-PCR area, and paleogenetic labs, which are located in a separate building. We use the following molecular genetic approaches.

  • DNA isolation of environmental samples and organismic material
  • PCR & quantitative PCR
  • Metabarcoding
  • Metagenomics
  • DNA hybridization capture
  • Preparation for amplicon and shot gun Next-Generation sequencing
  • Cloning and preparation for Sanger sequencing
  • Microsatellites – SNP analyses

Peculiarity of paleo genetic laboratory

  • Separation from the general genetic labs preventing contamination risks
  • High degree of purity (UV ceiling lights, separation in pre- and main rooms)
  • Isolation of ancient DNA from ancient samples (e.g. lake sediment, permafrost)
  • DNA library preparation of ancient DNA for shot gun sequencing

Selection of lab equipment

  • UV workstations
  • Fast-Prep®-24 Instrument
  • End-point PCR cycler & RotorGene qPCR cycler
  • Covaris M220 Focused-ultrasonicator™
  • Qubit 4 Fluorometer & 4200 TapeStation

Lead
Dr. Kathleen Stoof-Leichsenring

Technical staff
Sarah Olischläger
Janine Klimke

Technology

The laboratories are among others equipped for the following approaches: