Microscopy

As one of the central interfaces, our microscopy laboratory allows the transfer of real microstructures into the virtual world.The confocal laser scanning microscope displays the marine plankton in four dimensions with a resolution of less than 200 nm. With the help of micromanipulators and self-manufactured and calibrated micropipettes, the smallest forces can be measured in fracture tests and visualized by high-speed camera. With the help of these data, computer simulations can be performed and validated in our working group.For the isolation and analysis of plankton organisms, e.g. from fresh net catches in front of the house door, stereo magnifiers with colour cameras are available.

Equipment

  • Confocal laser scanning microscope Olympus FV-10i
  • Reversing microscope Zeiss AxioObserver A1, expandable with two Sutter Instruments MPC-285 micromanipulators
  • Femtotools FT-RS1002 Microrobotic System with micro force sensors
  • Fastcam-X 1024 PCI high speed camera (1000fps/fullframe up to 109500fps)
  • Micropipette puller Sutter P97
  • De Fonbrune Microforge
  • Olympus SZX10 binocular microscope with ColorView camera
  • Olympus SZX12 binocular microscope
  • Olympus SZX16 binocular microscope with XC10 camera and MW Tango USB motorized z-stage

Methods

  • Different fluorescence colouring techniques for CLSM analysis
  • 3D imaging techniques, -analysis and -export with Bitplane Imaris 7.3 software
  • Specially manufactured micropipettes for micro-crash tests
  • Force response curves for compression/micro crash tests with Femtotools FT-RS1002 Microrobotic System
  • High-speed imaging (1000fps@1024x1024px, 109,500fps@128x16px) with Zeiss Axioobserver A1 microscope, up to 1000x magnification
  • Focused images and 3D surface reconstruction with the Olympus SZX16 and motorized Z-drive